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脂肪安排应对反响提醒了脂肪生成在猪习惯热应激过程中的重要作用

2018-8-1 10:27| 发布者: 畜牧修改| 检查: 2347| 谈论: 1|原作者: 畜牧修改

  第145期:脂肪安排应对反响提醒了脂肪生成在猪习惯热应激过程中的重要作用
  J. Anim. Sci. 2018.96:975–989
  原文链接:
  DOI: 10.1093/jas/sky022


  脂肪安排应对反响提醒了脂肪生成在猪习惯热应激过程中的重要作用
  H. Qu, and K. M. Ajuwon
  翻译: 王帅  校正: 上海亘泰实业集团
  外界温度升高会引起猪热应激,下降动物出产功能。本实验的研讨意图是探求猪热应激时发生的安排应对反响。

  将成长猪随机分为4个处理组:急性热应激组,35 ± 1 oC处理24 h;缓慢热应激组,35 ± 1 oC处理7天;对照组,20 ± 1 oC处理7天;配对饲喂组,温度处理同对照组,依照缓慢热应激组的采食量给予饲料。除配对饲喂组外,其他处理组均自在采食。


  与对照组比较,缓慢热应激和急性热应激形成采食量别离下降36%和64%。与缓慢热应激组比较,急性热应激组成长猪血浆中游离脂肪酸浓度明显添加(P = 0.031)。配对饲喂组成长猪血浆中的胰岛素含量明显低于对照组(P =0.045)。与对照组比较,缓慢热应激组成长猪血浆尿素氮含量明显进步(P = 0.008),而急性热应激组成长猪血浆尿素氮含量明显低于缓慢热应激组(P < 0.021)。缓慢热应激组成长猪皮下脂肪安排中PCK1的mRNA和蛋白表达水平明显高于对照组和配对饲喂组(P < 0.05),一起也明显高于急性热应激组(P < 0.05)。虽然急性热应激组成长猪皮下脂肪安排中GK的mRNA表达水平低于缓慢热应急组(P = 0.003),但GK的mRNA表达水平在对照组、配对饲喂组和缓慢应急组之间差异不明显。配对饲喂组成长猪皮下脂肪安排中的内质网应激符号物-增强子结合同源蛋白的蛋白表达水平明显高于对照组(P < 0.05),缓慢热应激组中增强子结合同源蛋白的蛋白表达水平高于急性热应激组(P = 0.033)。缓慢热应激组肠系膜安排中PCK1的mRNA表达水平明显高于对照组和配对饲喂组(P < 0.001),急性热应激组中PCK1的mRNA表达水平明显低于缓慢热应激组(P = 0.039)。配对饲喂组成长猪肝脏中PCK1的表达水平明显低于其他处理组(P < 0.05),而肝脏和肌肉安排中其他检测目标在各处理组之间差异不明显。

  实验成果证明,热应激可以诱导激烈的脂肪安排反响,有利于添加脂肪贮存,表明晰脂肪安排可能在猪习惯热应激过程中起着重要作用。

  关键词:脂肪安排、增强子结合同源蛋白、热应激、磷酸烯醇丙酮酸羧激酶、猪

  Adipose tissue-specific responses reveal an important role of lipogenesis during heat stress adaptation in pigs

  H. Qu, and K. M. Ajuwon

  Elevated ambient temperature causes heat stress in pigs, resulting in reduced animal performance. To better understand tissue responses to heat stress in pigs, we conducted a study in which pigs were subjected to four treatments: acute (24 h) heat stress (AHS) at 35 °C ± 1 ambient temperature, chronic (7 d) heat stress at 35 °C ± 1 (HS) or normal ambient temperature (20 °C± 1) for 7 d with ad-libitum feeding (Con) or with pair-feeding to the feed intake (FI) of the HS pigs (PF). Heat stress decreased FI by approximately 36% and 64% in HS and AHS treatments respectively, compared with Con (P < 0.01). Concentration of free fatty acids (FFA) was elevated in AHS compared to HS (P = 0.031). Serum insulin concentration was lower in PF than Con (P = 0.045). Blood urea nitrogen (BUN) concentration was elevated in HS compared with Con and PF (P = 0.008), but lower (P < 0.021) in AHS compared to HS. In the subcutaneous adipose tissue, the mRNA and protein abundance of PCK1 were higher (P < 0.05) in the HS treatment than Con and PF, and also higher (P < 0.05) in HS than AHS. However, there was no difference in GK mRNA between Con, PF, and HS, although its expression was lower(P = 0.003) in AHS vs. HS. Protein abundance of the ER stress marker, CCAT/enhancer-binding homologous protein (CHOP), was higher in PF than Con (P< 0.05), and higher (P = 0.033) in HS than AHS in subcutaneous fat. Inmesenteric fat, PCK1 mRNA was higher (P < 0.001) in the HS than Con and PF treatments. Additionally, expression of PCK1 was lower (P = 0.039) in AHS vs. HS. Expression of PCK1 was down regulated (P < 0.05) in the liver of PF pigs compared to other treatments, but most other genes measured were not affected by treatment in the liver and muscle tissues. These results confirm that heat stress induces a robust adipose tissue response in favor of increased lipids torage. This indicates that adipose tissue might play an important role in heat stress adaptation.

  Key words: adipose tissue, CHOP, heat stress, PCK1 or PEPCK, pig



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引证 bonderic 2018-8-28 20:12
热应激可以诱导激烈的脂肪安排反响,有利于添加脂肪贮存。这个定论不太契合咱们日常认知。

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